Cultivo de islotes de Langerhans, diferenciados de células Beta y su relación con ISO y ALO transplantes.
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Palabras clave

Cultivo de islotes de Langerhans
células BETA
ISO transplantes
ALO transplantes

Cómo citar

Sánchez Medina, M., Rodríguez N., B. M., & de García, R. (2018). Cultivo de islotes de Langerhans, diferenciados de células Beta y su relación con ISO y ALO transplantes. Revista Colombiana De Endocrinología, Diabetes &Amp; Metabolismo, -12(2), 80–108. https://doi.org/10.53853/encr.-12.2.170

Resumen

Se cultivaron islotes betacelulares pancreáticos de ratones, curies, ratas, hamsters y conejos, para estudiar los factores que influencian el crecimiento y desarrollo de cultivo "in-vitro". No se usaron hormonas. El medio de cultivo fue líquido ascítico, suero de gallo y solución de Tyroide. Los islotes desarrollados se diferenciaron e identificaron en el momento del explante. Al vigésimo día se observaron notoriamente betacélulas granuladas. Se demostró un continuo mantenimiento de las células alfa, beta y delta a partir del décimosexto día, el cual era paralelo con la regresión del conglomerado acinar. La masa total del explante disminuyó mientras que la masa del islote permaneció relativamente sin modificacion. Las betacélulas mostraron una continua estabilización, de acuerdo con el modelo animal experimental. Las vacuolas presentes de diferentes tamaños sugieren una posible actividad beta celular. Nucléolos muy claros se observaron en las células y numerosos gránulos en el citoplasma. Las betacélulas granuladas mostraron gránulos citoplasmáticos muy claros por medio de diferentes coloraciones. Una posible relación embriogénica entre el conglomerado de las betacélulas en el cultivo y los elementos morfológicos posiblemente vasculares, se plantea como una factible hipótesis en consideración.

SUMMARY

CULTURES OF THE ISLETS OF LANGERHANS, BETA-CELL DIFFERENTIATES, ANDTHEIR RELATION TO ISO AND ALLO TRANSPLANTS.

Cultured cells from pancreatic islets from normal rats, quinea ping, hamsters and rabbits were utilized to study the factors influencing beta-cell culture in vitro. No hormones were used. The culture medium was asciitic fluid, rooster serum and Tyrode's solution. Developed differentiated islets were identified at the time of the explant. On the 20th day, remarkable granulated cells were seen. A continuous maintenance of the beta, alpha and delta cells was observed after the 16th day along with regression of the acinar cells. The total mass of the explant decreased while the islet mass was relatively unchanged. The beta cells showed a stable and permanent vital state in the culture, according with the experimental animal model. Vacuoles of different sizes showed a possible activity of the beta cells and large numbers of beta granules in the cytoplasm were seen. Distinct nucleoli were observed. The granulated beta cells showed clear cytoplasmic granules in several stains. A possible embryogenic relation between the conglomeration of beta cells in the culture and the morphologic elements of the vascular wall was prominent.



 

https://doi.org/10.53853/encr.-12.2.170
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Citas

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