Testicular histopathology of rats BIOU: Wistar exposed to malathion
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endocrine disruptors

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Serrano, R., Hernández, G. A., Hung, S., Lozano, R., Paoli, M., & Gómez, R. (2019). Testicular histopathology of rats BIOU: Wistar exposed to malathion. Revista Endocrino, 6(4), 252–259. https://doi.org/10.53853/encr.6.4.544


Objective: To determine the histopathology of the testes of BIOU: Wistar rats chronically exposed to malathion compared to non-exposed rats.
Methods: Experimental study with 20 male rats randomized, 10 to the control group and 10 to the exposed group. The exposure consisted in the chronic application of inhaled malathion once a week for 2 hours for 14 weeks, with equivalent doses to those used by humans in agriculture. The control group was exposed to a placebo (distilled water) in the open air for the same interval time. Afterwards, they were sacrificed, a serum sample was obtained for the determination of acetylcholinesterase and the testicles were extracted for histopathological study.
Results: Acetylcholinesterase levels were significantly lower (p = 0.0001) in the exposed group than in the control group. In the histopathological study with light microscopy, in the exposed group, vacuolation of the germinal epithelium was observed in 50 % of the cases (p = 0.016) for an odds ratio of 3.00 (95 %:1.46- 6.13), as well as incomplete spermatogenesis in 40 % (p = 0.043) for an odds ratio of 2.66 (95 %:1.41-5.02), while there were no alterations in the control group. There was a significant inverse correlation (p = 0, 0001) between the number of histopathological alterations and acetylcholinesterase activity.
Conclusions: These results show that chronic exposure to malathion in BIOU: Wistar rats can produce deleterious effects on their reproductive system.

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