Keywords
triyodotironina
radioinmunoanálisis
columnas de Sephadex G-25
radioinmunoanálisis
columnas de Sephadex G-25
How to Cite
Arango R., G., & Wahner, H. W. (2018). Un método sencillo para la medición de la triyodotironina en suero por radioinmunoanálisis utilizando columnas de Sephadex G-25. Revista Colombiana De Endocrinología, Diabetes &Amp; Metabolismo, -10(1), 14–21. https://doi.org/10.53853/encr.-10.1.201
Abstract
Fragmento
Un nuevo método para la medición de la triyodotironina (T3) en suero ha aparecido recientemente en el comercio; puede efectuarse en un corto período de tiempo, aproximadamente 4 horas, en cualquier laboratorio equipado con un contador de pozo para rayos gamma. El método es muy similar al que se utiliza para la tiroxina sérica, ampliamente conocido. Ambos procedimientos están al alcance prácticamente de cualquier médico interesado en el diagnóstico de las enfermedades de la tiroides.
References
1. Sterling K.: The significance of circulating triiodothyronine. Rec. Pro. Horm. Res. 26:249-275, 1970.
2. Hollander, C.S.; On the nature of the circulating thyroid hormone: Clinical studies of triidothyronine and thyroxine in serum using gas chromatographic methods. Trans. Ass. Amer. Physicians. Philadelph a 81:76, 1968.
3. Ballabarba, D.: Further observations on T3 thyrotoxicosis (Abstract). Clin. Res. 20:421, 1972.
4. Hollander, C.S., et al: T3 toxicosis: Association with iodine deficiency (Abstract). Clin. Res. 20: 263, 1972.
5. Gaitán, J. E., et al: Importancia de la medición de triyocotironna en suero como prueba de evaluación tiroidea. Rev. Soc. Colomb. Endocrinol. IX (1): 29.41, July 1973.
6. Gaitán, J. E. et al: Importancia de la medición de triyodotironina y de T4 unica a la proteína en suero como pruebas de evaluación tiroidea. Acta Med. Valle. 4:8-13. 1973.
7. Larsen, P. R.: Direct immunoassay of triiodothyronine in human serum. J. Clin. Invest. 51:1939-1949, August 1972.
8. Surks, M. I., et al: A new radioimmunossay for plasma L-triiodothyronine: Measurements in thyroid disease and in patients maintained on hormonal replacement. J. Clin. Invest. 51:3104.3113, Dec. 1972.
9. Gharb, H., et all : Radioimmunoassay for triiodothyronine (T3): I. Affinity and specificity of the antibody for T3. J. Clin. Endocrinol. Met. 33:509, 1971.
10. Chopra, I. J., et al: Radioimmunoassay for measurament of triiodothyronine in human serum. J. Clin. Invest 50: 2033-2041, 1971.
11. Mitsuma, T., et al: Serum triiodothyronine: Measurement in human serum by radiommunoassay with corroboration by gas-liquid chromatography J. Clin. Invest. 50:2679.26-68, 1971
12. Lieblich, H. J., and R. D. Utiger: Triiodothyronine radioimmunoassay. J. Clin. Invest. 51:157, 1972.
13. Guansing, A. R., et al: Production of specific antibodies against triiodothyronine (T3) (Abstract). Clin. Res. 19:772, 1971.
14. Gharib, H., et al: Triiodothyronine (T3) radioimmunoassay. A critical evaluation. Mayo Clin. Proc. 47:934.937, 1972.
15. Wahner, H. W., and Colum A. Gorman: Interpretation of serum triodothyronine levels measured by the Sterlin technic. New Engl. J. Med. 284:225, 1971.
2. Hollander, C.S.; On the nature of the circulating thyroid hormone: Clinical studies of triidothyronine and thyroxine in serum using gas chromatographic methods. Trans. Ass. Amer. Physicians. Philadelph a 81:76, 1968.
3. Ballabarba, D.: Further observations on T3 thyrotoxicosis (Abstract). Clin. Res. 20:421, 1972.
4. Hollander, C.S., et al: T3 toxicosis: Association with iodine deficiency (Abstract). Clin. Res. 20: 263, 1972.
5. Gaitán, J. E., et al: Importancia de la medición de triyocotironna en suero como prueba de evaluación tiroidea. Rev. Soc. Colomb. Endocrinol. IX (1): 29.41, July 1973.
6. Gaitán, J. E. et al: Importancia de la medición de triyodotironina y de T4 unica a la proteína en suero como pruebas de evaluación tiroidea. Acta Med. Valle. 4:8-13. 1973.
7. Larsen, P. R.: Direct immunoassay of triiodothyronine in human serum. J. Clin. Invest. 51:1939-1949, August 1972.
8. Surks, M. I., et al: A new radioimmunossay for plasma L-triiodothyronine: Measurements in thyroid disease and in patients maintained on hormonal replacement. J. Clin. Invest. 51:3104.3113, Dec. 1972.
9. Gharb, H., et all : Radioimmunoassay for triiodothyronine (T3): I. Affinity and specificity of the antibody for T3. J. Clin. Endocrinol. Met. 33:509, 1971.
10. Chopra, I. J., et al: Radioimmunoassay for measurament of triiodothyronine in human serum. J. Clin. Invest 50: 2033-2041, 1971.
11. Mitsuma, T., et al: Serum triiodothyronine: Measurement in human serum by radiommunoassay with corroboration by gas-liquid chromatography J. Clin. Invest. 50:2679.26-68, 1971
12. Lieblich, H. J., and R. D. Utiger: Triiodothyronine radioimmunoassay. J. Clin. Invest. 51:157, 1972.
13. Guansing, A. R., et al: Production of specific antibodies against triiodothyronine (T3) (Abstract). Clin. Res. 19:772, 1971.
14. Gharib, H., et al: Triiodothyronine (T3) radioimmunoassay. A critical evaluation. Mayo Clin. Proc. 47:934.937, 1972.
15. Wahner, H. W., and Colum A. Gorman: Interpretation of serum triodothyronine levels measured by the Sterlin technic. New Engl. J. Med. 284:225, 1971.
Authors must state that they reviewed, validated and approved the manuscript's publication. Moreover, they must sign a model release that should be sent. A copy may be reviewed here